These are streptavidin conjugated Hi-Sur Mag beads with diameter of 150 nm. The magnetic beads are pre-blocked by a layer of BSA before a monolayer of recombinant streptavidin covalently coupled to the surface. The monolayer of streptavidin makes most of the biotin binding sites sterically available for binding of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The 150 nm Hi-Sur Mag Streptavidin Beads have a large surface area, high capacity, efficient magnetic pull, and slow sedimentation rate during incubation. 150 nm Hi-Sur Mag Streptavidin Beads have higher binding capacity than 150 nm Super Mag due to their large surface area.
- Higher binding capacity than other 150 nm streptavidin conjugated magnetic beads
- Significant low non-specific binding to proteins and nucleic acid
- Fast magnetic separation
Ideal for isolation of cell separation and assay development. Low non-specific binding of proteins and nucleic acids.
One mg of 150 nm Hi-Sur Mag Beads typically binds:
- >7,000 pmoles free biotin
- ~1200 pmol biotinylated peptides
- >60 µg biotinylated IgG
- ~60 µg ds-DNA
- ~1400 pmol ss-oligonucleotides
Product Description: Streptavidin Conjugated Superparamagnetic Beads with Large Surface Area
Solvent: 10 mM PBS, 2 mM EDTA, 0.05% NaN3, 0.01% tween 20, 0.5% BSA, pH 7.4
Concentration: 1 mg/mL
Particle Size by Dynamic Light Scattering: 140-180 nm
Surface Ligands: Streptavidin
Magnetic Content: >70%
Number of Beads in 1 mL: 4×1012
Storage: 2-8 °C; Do not freeze. Keep vial upright.