P0211 - PureBind Swab Genomic DNA Isolation Kit - Nucleic Acid Isolation Kits - By Applications
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P0211

Details

High Molecular Weight Genomic DNA: High yields of high molecular weight genomic DNA isolated from buccal cell swabs with the PureBind Swab gDNA kit. Donor buccal cells collected on Puritan® HydraFlock® swabs were processed through the PureBind Swab kit and eluted in 60 µL. 500 nanograms of purified DNA was electrophoresed on a 1% agarose gel/TAE alongside the GeneRuler™1kb Plus ladder (Thermo Scientific); uppermost DNA ladder band is 20,000 bp.

High Purity Genomic DNA from Buccal Swabs: High purity genomic DNA isolated from buccal cell swabs with the PureBind Swab gDNA kit. Donor buccal cells collected on Puritan® HydraFlock® swabs were processed through the PureBind Swab kit and eluted in 60 µL. Eluted DNA was analyzed by spectrophotometric analysis to determine purity ratios and DNA concentration.

Comparison to Other Commercial Swab Kits: PureBind Swab gDNA kit outperforms competitive products for DNA yield and purity from buccal swab extractions. 12 Donor buccal swabs collected on Puritan® HydraFlock® swabs/kit were processed through the PureBind Swab kit and each competitive kit according to the manufacturer’s instructions. Eluted DNA was analyzed via NanoDrop for concentration and spectrophotometric purity. (Data courtesy of Aaron Chen and Ryan Cedeno, Genenius Genetics).

NGS Library Prep with PureBind Swab-isolated genomic DNA: High quality NGS library preparation with genomic DNA isolated with the PureBind Swab gDNA kit. Donor buccal cells collected on Puritan® HydraFlock® swabs were processed through the PureBind Swab kit and eluted in 60 µL. Purified DNA was used in a 1400 amplicon multiplex-PCR target enrichment alongside a control genomic DNA preparation from Roche. Amplicons were sequenced on an Illumina NextSeq550 (mid output, 300 cycles, dual index, PE150 mode) and analyzed for read quality relative to the Roche control gDNA. Due to the inherent bacterial DNA fraction in swab samples the library yield and usable reads are slightly lower than the Roche control but this does not affect the percentage of amplicons passing QC. (Data courtesy of Aaron Chen and Ryan Cedeno, Genenius Genetics).

Catalog# Product Unit Size Unit Price Quantity
P0211-S PureBind Swab Genomic DNA Isolation Kit 1 mg $0.00
P0211-96 PureBind Swab Genomic DNA Isolation Kit 1 µm $299.00
P0211-384 PureBind Swab Genomic DNA Isolation Kit 10 nm $1,017.00
Product Description PureBind Swab Genomic DNA Isolation Kit
Application Swab Genomic DNA Isolation