P0311 - NuPure PCR Cleanup Kit - Nucleic Acid Isolation Kits - By Applications
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P0311

Details

Strong recovery of DNA products ≥ 100 bp: Efficient exclusion of DNA fragments < 100 bp; increased retention of DNA ≥ 100 bp. 50 µL (50 ng/ µL) of DNA ladder was processed with 1.8x AMPure XP vs. NuPure beads. The beads were washed and bound DNA was eluted and electrophoresed on a 2.5% agarose gel/TBE alongside the GeneRuler™50 bp ladder (Thermo Scientific). Both PCR clean up platforms exclude the 50 bp band but NuPure has greater retention of most fragments ≥ 100 bp.

Effective removal of PCR reactants and primer-dimer side products by NuPure but not by spin column kits: NuPure removal of an 56 bp primer-dimer that is retained by spin column PCR clean up kits. Using bacterial 16S rRNA PCR amplification as a model system, low level template input (103 fg, 196 genome equivalents) leads to a competing side reaction that results in discernible primer-dimer formation (denoted with a single asterisk). NuPure PCR Clean Up is able to completely remove this primer dimer while retaining the desired 470 bp amplicon product. Note: ** denotes a non-DNA gel artifact that does not appear in the corresponding BioAnalyzer data. 2.5% agarose gel/TBE alongside the GeneRuler™50 bp ladder (Thermo Scientific).

BioAnalyzer analysis - NuPure delivers high yields of fragments ≥ 100 bp: Efficient exclusion of DNA fragments < 100 bp; increased retention of DNA ≥ 100 bp. 50 µL (50 ng/ µL) of DNA ladder was processed with 1.8x AMPure XP vs. NuPure beads. The beads were washed and bound DNA was eluted in 50 µL (same volume as the original input ladder) and analyzed via the BioAnalyzer (A). Integrated peak intensities from the GeneRuler 50 bp Ladder, AMPure XP and NuPure were used to calculate the % recovery of input ladder DNA (B).

BioAnalyzer analysis of NuPure vs. Spin Column Kits: BioAnalyzer analysis of PCR clean up reactions demonstrating retention of the  56 bp primer-dimer by spin column PCR clean up kits. (A) NuPure purified PCR product showing one 16S amplicon product peak. (B) QIAquick retention of the primer-dimer, indicated with *. (C) Zymo DNA Clean & Concentrate retention of the primer-dimer, indicated with *.

Effective removal of PCR reactants and primer-dimer side products by NuPure but not by ChargeSwitch®: NuPure removal of an 56 bp primer-dimer that is retained by charge-based PCR clean up kit. Using the bacterial 16S rRNA PCR amplification model system, 1000 fg of E.coli genomic DNA was amplified. As noted before, this low level template reaction leads to discernible primer-dimer formation (denoted with a single asterisk). NuPure PCR Clean Up is able to completely remove this primer dimer while retaining the desired 470 bp amplicon product. Note: ** denotes a non-DNA gel artifact that does not appear in the corresponding BioAnalyzer data. 2.5% agarose gel/TBE alongside the GeneRuler™50 bp ladder (Thermo Scientific).

Effective removal of PCR reactants and primer-dimer side products by NuPure but not by Mag-Bind® SeqDTR™: NuPure removal of an  56 bp primer-dimer that is retained by Mag-Bind SeqDTR chemistry. Using the bacterial 16S rRNA PCR amplification model system, 1000 fg of E.coli genomic DNA was amplified and subjected to PCR clean up reactions. NuPure PCR Clean Up is able to completely remove the 56 bp primer-dimer (*) while retaining the desired 470 bp amplicon product. Note: ** denotes a non-DNA gel artifact. 2.5% agarose gel/TBE alongside the GeneRuler™50 bp ladder (Thermo Scientific).

NuPure Clean Up of Plasmid Restriction Digests: NuPure purification of restriction digested plasmid. 2713 bp pUC19 plasmid was digested with KasI, BstAPI and with a KasI/BstAPI combination. After digestion, the 50 µL reaction was purified with NuPure PCR Clean Up and analyzed via 1.0% agarose gel/TAE alongside the 1 kb Plus DNA ladder (Thermo Scientific).

Catalog# Product Unit Size Unit Price Quantity
P0311-SMP NuPure PCR Cleanup Kit 1 mg $0.00
P0311-096 NuPure PCR Cleanup Kit 1 µm $99.00
P0311-384 NuPure PCR Clean Up Kit 15 nm $337.00
Product Description NuPure PCR Clean Up Kit
Application PCR Clean Up