Q: What is the coating on the water soluble SHP iron oxide nanocrystals?
A: The coating of our SHP iron oxide nanocrystals includes monolayer oleic acid and monolayer amphiphilic polymer.
Q: What is the overall size of your water soluble SHP iron oxide nanocrystals?
A: The oleic acid coating and the polymer coating are approximately 2 nm in thickness respectively. They together add approximately 8 nm to the overall core size.
Q: How is the stability of the SHP iron oxide nanocrystals in buffer solutions?
A: SHP iron oxide nanocrystals are stable in most buffer solutions, for example PBS, HEPES, Tris, borate, etc. except for in MES. The concentration of the buffer solution can be up to 1 M. We haven’t fully tested the stability in buffer solutions which concentration is more than 1 M.
Q: What is the stability of your SHP iron oxide nanocrystals under pH and salt conditions?
A: Our SHP iron oxide nanocrystals are stable in the pH range of 5-10 and can survive in salt when the concentration of the salt is below 1 M (as tested with NaCl). We haven’t fully tested the stability in salt at higher concentrations.
Q; How can I test the stability of your SHP iron oxide nanocrystals in water?
A: You can run electrophoresis gel. Our SHP iron oxide nanocrystals should show a single band in the gel.
Q: What is the concentration of iron in your SHP iron oxide nanocrystal solutions?
A: The standard concentration of iron in our products is 5 mg/ml (Fe). However, this concentration can be adjusted upon customers’ request.
Q: What is the storage solution for your SHP iron oxide nanocrystals?
A: It’s in double distilled water.
Q: What is the shelf life of your SHP iron oxide nanocrystals in water?
A: The shelf life of our SHP iron oxide nanocrystals depends on the size of the crystals. The shelf life of iron oxide nanocrystals less than 15 is 1 year, if they are stored and handled correctly. We have been manufacturing our 10 nm iron oxide nanocrystals for over two years and have not yet seen a definitive lifetime. The shelf life of our iron oxide nanocrystals larger than 15 nm is 6 months if they are stored and handled correctly.
Q: How can I conjugate protein to SHP nanocrystals?
A: EDAC coupling method can be used to conjugate protein onto the surface of our SHP iron oxide nanocrystals. Please check the EDAC conjugation protocol in the “Conjugation Protocol” section under “Products” or “Resource” tabs.
Q; How can I determine whether the protein is conjugated on to the surface of your SHP iron oxide nanocrystals?
A: You can run electrophoresis gel. The protein conjugated Iron oxide nanocrystals should migrate slower than the original SHP iron oxide nanocrystals.
Q: How do I calculate the molar amount of 1 mg Fe for different sized SHP iron oxide nanocrystals?
A:
|
Crystal Size (nm)
|
Nanocrystal Molar Concentration (nmol) of 1 mg Fe
|
Nanocrystal Molar Concentration (nmol) for 10 mg Fe
|
Nanocrystal Molar Concentration (nmol) for50 mg
|
|
5
|
6.9
|
69
|
345.0
|
|
10
|
0.86
|
8.6
|
43
|
|
15
|
0.27
|
2.7
|
13.5
|
|
20
|
0.11
|
1.1
|
5.5
|
|
25
|
0.058
|
0.58
|
2.9
|
|
30
|
0.034
|
0.34
|
1.7
|
|
35
|
0.021
|
0.21
|
1.05
|
|
40
|
0.014
|
0.14
|
0.7
|
|
45
|
0.010
|
0.1
|
0.5
|
|
50
|
0.0073
|
0.073
|
0.365
|
Q: Why do you use Fe amount as unit?
A: We use x-ray fluorescent spectra to quantify the concentration of Iron. Thus, we can only have the information about iron.
Q: Can I use a magnetic separator to purify the SHP iron oxide nanocrystals after they are conjugated with biomolecules?
A: Yes, you can. You have to use the magnetic separator we have specifically designed to separate the crystals larger than 10 nm. The commercial magnetic separator is for the separation of magnetic beads larger than 100 nm. Their magnetic gradient is not enough to separate our iron oxide nanocrystals smaller than 100 nm.
Q: How do I purify the iron oxide nanocrystals to remove the reaction regents and the excess protein after conjugation?
A: You can use our specifically designed magnetic separator SuperMag SeparatorTM to purify if sizes are larger than 10 nm. Q: What is the coating on your SHA water soluble iron oxide nanocrystals?
A: Our SHA iron oxide nanocrystals are coated with monolayer oleic acid, monolayer amphiphilic polymer and monolayer of PEG
Q: How is the stability of your SHA iron oxide nanocrystals in buffer solutions?
A: SHP iron oxide nanocrystals are stable in most buffer solutions, for example PBS, HEPES, Tris, borate, etc. except for in MES. The concentration of the buffer solution can be up to 1 M. we haven’t fully tested their stability the buffer solutions which concentration is more than 1 M.
Q: What is the storage solution for your SHA iron oxide nanocrystals?
A: pH 5.0 20 mM borate buffer solution.
Q: What is the concentration of iron in your SHA iron oxide nanocrystal solutions?
A: The standard concentration of iron in our SHA iron oxide nanocrystal solutions is 1 mg/ml (Fe). However, this concentration can be adjusted upon customers’ request.
Q: How can I conjugate protein onto your SHA iron oxide nanocrystal?
A: Trout’s agent can be used to conjugate the SHA iron oxide nanocrystals with amine surface and thiol derived biomolecules.
Q; How can I determine whether protein is conjugated onto the surface of the SHA iron oxide nanocrystals?
A: You can run electrophoresis gel. The protein conjugated Iron oxide nanocrystals should migrate faster than the original SHA iron oxide nanocrystals.
Q: can I use magnetic separator to purify the SHA iron oxide nanocrystals after they are conjugation with biomolecules?
A: Yes, you can, however, the commercial magnetic separator is for separating magnetic beads for sizes larger than 100 nm, their magnetic gradient is not enough to separate our iron oxide nanocrystals. Ocean Nanotech has a specifically designed magnetic separator which can separate the crystal size larger than 10 nm iron oxide nanocrystals.
Q: How do I purify the iron oxide nanocrystals to remove the reaction regents and the excess protein after conjugation?
A: You can use our specially designed magnetic separator SuperMag SeparatorTM to purify if sizes are larger than 10 nm. 
